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25 items in this album on 3 pages | [slideshow] [login] | |
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Gallery: Zach's Bee Photos [(c) Zachary Huang], for Prints  Album: Bee research
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| Equipment/Supplies (1)
70% alcohol for cleaning fingers and wiretrol. Wax dishes for fixing bees. A good ruler for measuring length of hemolymph. Viewed: 1761 times. | |||||||||
| At least one insect pin, which is bent so that it is easier to orient to the bee body axis. Viewed: 1626 times. | |||||||||
| Equipment/Supplies (2). We use this type of modelling clay for fixing bees. Available from most hobby stores. Viewed: 1600 times. | |||||||||
| Equipment/Supplies (3). We use this type of acetonitrile from EM Science. Any brand would work as long as they are HPLC grade or higher. Viewed: 1639 times. | |||||||||
| Equipment/Supplies (4). We use Drummond wiretrol capillary tubes. Each 100 tubes come with one plunger (with the white handle). The tubes are marked for 1-5 microliters. However, because we bake all glassware that come into contact with hemolymph or JH, the marks do not stay. We use a ruler to measure the length of blood in millimeters instead. Make sure you know 5 microliters = how many millimeters. In this case, 5 ul = 27 mm, so we know later how convert mm back to ul (1 ul = 5.4 mm). Viewed: 1747 times. | |||||||||
| Equipment/Supplies (5)
We use 12 x 125 mm cultures like this. The most important thing is that the lids MUST be Teflon lined. Teflon is resistant to chemical solvents and also JH is less likely to stick to it. Never use plastic tubes or lids with plastic lining. Viewed: 1727 times. | |||||||||
| Equipment/Supplies (6):
You need a compound scope for pricking the hole and drawing blood. You do not need a high quality one (as shown here), simply 5-7 x magnification to see clearly. I have used naked eyes to do the whole process, but it is very hard on the eyes. Viewed: 1632 times. | |||||||||
| Put 500 ul of acetonitrile into each tube and cap the tube tightly. Viewed: 1582 times. | |||||||||
| Clearly label each tube before the blood is put in. Otherwise you will easily forget to label it after blood is put in and your sample is wasted. Viewed: 1653 times. | |||||||||
| We use an insect vacuum to collect bees from a colony. This photo shows the entrance is blocked with a piece of hardware cloth and the vacuum is used to collect returning foragers. Viewed: 1757 times. | |||||||||
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Gallery: Zach's Bee Photos [(c) Zachary Huang], for Prints Album: Bee research
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